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Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:
Ral-GTP
Cat. #: 26913 |
Size: 30 µL |
Gene Symbol: Ral |
Description: Anti-Ral-GTP Mouse Monoclonal Antibody |
Background: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation. |
Immunogen: Recombinant full length protein of active RalB. |
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Ral-GTP monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033). |
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Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase