Rab5-GTP

26911

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Rab5-GTP

Cat. #: 26911

Size: 30 µL

Gene Symbol: RAB5

Description: Anti-Rab5-GTP Monoclonal Antibody

Background: Rab5 is a member of Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

Immunogen: Recombinant full length protein of active Gao

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rab5 antibody recognizes active Rab5 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

Rab5 Activation Assay.
Purified full-length Rab5A proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rab5-GTP monoclonal antibody (Cat. No. 26911). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21151).

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Rab5-GTP

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