Rab35-GTP

26922

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Rab35-GTP

Cat. #: 26922

Size: 30 µL

Gene Symbol: RAB35

Description: Anti-Rab35-GTP Monoclonal Antibody

Background: The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.

Immunogen: Recombinant full length protein of active Rab35

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

Rab35 Activation Assay.Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).

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Product Targets

Product Categories

Rab35-GTP

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