Rab3-GTP

26920

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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Rab3-GTP

Cat. #: 26920

Size: 30 µL

Gene Symbol: RAB3

Description: Anti-Rab3-GTP Mouse Monoclonal Antibody

Background: Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles.

Immunogen: Recombinant full length protein of active Rab3A

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-Rab3-GTP monoclonal antibody recognizes active Rab3 proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western blot:

Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab3-GTP monoclonal antibody (Cat. No. 26920). Immunoblot was with an anti-Rab3A polyclonal antibody (Cat. No. 21041).

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