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Rab11-GTP

26919

Size:30 μl Price:$ 495 Brand:NewEast Place of Origin:USA Immunogen:

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Rab11-GTP

Cat. #:   26919
Size:  30 µL
Gene Symbol:  RAB11
Description:   Anti-Rab11-GTP Mouse Monoclonal Antibody
Background:   Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.
Immunogen:   Recombinant full length protein of active Rab11
Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:    Purified from ascites
Preservative:  No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rab11-GTP monoclonal antibody recognizes active Rab11 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  

Rab11 Activation Assay. Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).
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