RhoA-GTP

26904

Size：30 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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RhoA-GTP

Cat. #: 26904

Size: 30 µL

Gene Symbol: RHOA

Description: Anti-RhoA-GTP Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca ²⁺sensitivity of smooth muscle contraction.

Immunogen: Recombinant full length protein of active RhoA

Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2b

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg ²⁺and Ca ²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western Blot:

IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007).

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RhoA-GTP

Rheb-GTP

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Cdc42 Pull-Down Activation Assay Kit

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