EGFR(G719A)

26115

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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EGFR(G719A)

Cat. #: 26115

Gene Symbol: EGFR/HER1/ERBB1

Description: Anti-EGFR(G719A) Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of G719A, human origin.

Tested Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR(G719A), but not wild type EGFR of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.

Western blot:

Western blot analysis of recombinant EGFR(G719A) and wild type proteins.

Purified His-tagged EGFR(G719A) protein (aa 702-987) and wild type protein (aa 702-987) were blotted with anti- EGFR(G719A) mouse antibody (cat. # 26115).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(G719A) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(G719A) plasmid (right column), then fixed and stained with anti-EGFR (G719A) monoclonal antibody (cat. # 26115).

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EGFR(G719A)

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