EGFR(G719S)

26083

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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EGFR (G719S)

Cat. #: 26083

Gene Symbol: EGFR

Description: Anti-EGFR(G719S) Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer.

Immunogen: A synthetic peptide from the internal region of human EGFR which includes the mutation of G719S.

Applications: ELISA, WB, IHC, IF

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:2000 IHC: 1:50-1:1000 IF: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG2b

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.

Western blot:

Western blot analysis of recombinant EGFR(G719S) and wild type EGFR proteins. Purified his-tagged EGFR (G719S) protein (aa 702-987) (Cat. #10214) and wild type EGFR protein (aa 702-987) (Cat. #10210) were blotted with anti-EGFR(G719S) mouse antibody (Cat. #26083). Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G719S) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G719S) plasmid (right column), then fixed and stained with anti- EGFR (G719S) monoclonal antibody (Cat. #26083).

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EGFR(G719S)

IDH2(R172G)

IDH2(R172W)

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IDH2(R172S)

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