EGFR(L861Q)

26077

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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EGFR(L861Q)

Cat. #: 26077

Gene Symbol: EGFR/HER1/ERBB1

Description: Anti-EGFR(L861Q) Mouse Monoclonal Antibody

Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).

Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L861Q, human origin.

Tested Applications: ELISA, WB, IF, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:200

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes EGFR(L861Q), but not wild type EGFR from vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing.

Western blot:

Western blot analysis of recombinant EGFR(L861Q) and wild type proteins. Purified His-tagged EGFR(L861Q) protein (amino acids 702-987, lane 2, Cat. # 10211) and corresponding wild type protein (lane 1, Cat. # 10210) were blotted with anti-EGFR(L861Q) mouse monoclonal antibody (Cat. # 26077). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004). Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(L861Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(L861Q) plasmid (right column), then fixed and stained with anti- EGFR(L861Q) monoclonal antibody (Cat. #26077).

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EGFR(L861Q)

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