ERBB2 mAb

26127

Size：100 μl Price：$ 239 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

ERBB2 Monoclonal Antibody

Cat. #: 26127

Gene Symbol: ERBB2/HER2/Neu/c-Neu

Description: Anti-ERBB2 Mouse Monoclonal Antibody

Background: The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important criteria in clinic evaluating of the progression of breast cancer.

Immunogen: A synthetic peptide from the C-terminal region of ERBB2, human origin

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:200

Concentration: 1mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Erb-B2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (Cat. #10208) was blotted with anti-Erb-B2 monoclonal antibody (Cat. #26127). Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

Reference citations

No data available

Product Targets

Product Categories

ERBB2 mAb

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

Contant Us