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SUMO-1 pAb

21236

Size:100 μl Price:$ 219 Brand:NewEast Place of Origin:USA Immunogen:

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SUMO-1 Polyclonal Antibody

Cat. #:  21236
Gene Symbol:  SUMO1
Description:   Anti-SUMO-1 Rabbit polyclonal Antibody
Background:   Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1.
Immunogen:   A synthetic peptide corresponding to residues near the C-terminal of human SUMO-1.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Detects SUMO-1 but not SUMO-2/3 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of over-expressed SUMO-1 proteins.

HEK293 cells were transfected with SUMO-1 (lane 2) and SUMO-2 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with Anti-SUMO-1 rabbit polyclonal antibody (Cat. # 21236).

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