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Gα13 pAb

21005

Size:100 μl Price:$ 219 Brand:NewEast Place of Origin:USA Immunogen:

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Gα13 Polyclonal Antibody

Cat. #:  21005
Gene Symbol:  Ga13
Description:   Anti-Gα13 Rabbit Polyclonal Antibody
Background:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Immunogen:   Recombinant full length Gα13 protein
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA 1:100-1:5000
WB 1:100-1:2000
IHC 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:   Purified from serum
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  13 polyclonal antibody recognizes Gα13 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of Gα13 proteins.

MEF whole-cell lysates were used for western blot with anti Gα13polyclonal antibody (Cat. # 21005).

Immunofluorescence of mouse embryonic fibroblast (MEF) cells. Wild-type MEF cells (top panel) and Gα13 double knock-out cells (Gα13 -/-, bottom panel) were fixed and stained with anti-Gα13 polyclonal antibody (Cat. # 21005). Arrows indicate the dorsal ruffle structure of one cell.

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