KIT(W557R)

26275

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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KIT(W557R)

Cat. #: 26275

Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR

Description: Anti-KIT(W557R) Mouse Monoclonal Antibody

Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism.

Immunogen: A synthetic peptide from the internal region of KIT which includes the mutation of W557R, human origin.

Applications: ELISA, WB, IF, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:25-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes W557R mutant, but not wild type KIT of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant KIT(W557R) and wild type proteins. Purified His-tagged KIT(W557R) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-KIT(W557R) monoclonal antibody (Cat. #26275). Immunofluorescence:

Immunofluorescence of cells expressing KIT proteins with Anti-KIT(W557R) antibody. HEK293T cells were transfected with pCDNA3-GFP-KIT (WT) plasmid (left column) or pCDNA3-GFP-KIT(W557R) plasmid (right column), then fixed and stained with Anti-KIT(W557R) monoclonal antibody (Cat. #26275).

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KIT(W557R)

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