NFE2L2(E79Q)

26336

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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NFE2L2(E79Q)

Cat. #: 26336

Gene Symbol: NFE2L2, NRF2

Description: Anti-NFE2L2(E79Q) Mouse Monoclonal Antibody

Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.

Immunogen: A synthetic peptide from the internal region of NFE2L2 which includes the mutation of E79Q, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:2000WB: 1:500-1:1000IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes E79Q mutant, but not wild type NFE2L2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant NFE2L2(E79Q) and wild type proteins. Purified His-tagged NFE2L2(E79Q) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-NFE2L2(E79Q) monoclonal antibody (Cat. #26336).Immunofluorescence:

Immunofluorescence of cells expressing NFE2L2 proteins with Anti-NFE2L2(E79Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-NFE2L2 (WT) plasmid (left column) or pCDNA3-GFP-NFE2L2(E79Q) plasmid (right column), then fixed and stained with Anti-NFE2L2(E79Q) monoclonal antibody (Cat. #26336).

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NFE2L2(E79Q)

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