RAS(G13R)

26474

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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RAS(G13R)

Cat. #: 26474

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS(G13R) Mouse Monoclonal Antibody

Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G13R mutant, but not wild type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant HRAS(G13R) and wild type proteins. Purified His-tagged RAS(G13R) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(G13R) monoclonal antibody (Cat. #26474). Immunofluorescence:

Immunofluorescence of cells expressing Ras proteins with Anti-RAS(G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13R) plasmid,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid, pCDNA3-GFP-Ras (G13C) plasmid, pCDNA3-GFP-Ras (G13V) plasmid, pCDNA3-GFP-Ras (G13A) plasmid, or pCDNA3-GFP-Ras (G13D) plasmid, then fixed and stained with Anti-RAS(G13R) monoclonal antibody (Cat. #26474).

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RAS(G13R)

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

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