SPP1 Rabbit pAb

210499

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S210499

Product Name: Anti-SPP1 Rabbit Polyclonal Antibody

Synonyms: OPN; BNSP; BSPI; ETA-1

UNIPROT ID: P10451 (Gene Accession - BC007016 )

Background: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: Fusion protein of human SPP1

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 25-100;WB: 500-2000;ELISA: 1000-2000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Cardiovascular, Signal Transduction, Stem Cells, Cancer

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 210499(SPP1 Antibody) at a dilution of 1/20(Cytoplasm).	In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 210499(Anti-SPP1 Antibody) at dilution 1/20.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-3: 293T, HepG2, U251 cell lysates; Primary antibody: 210499(SPP1 Antibody) at dilution 1/600; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 20 seconds

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SPP1 Rabbit pAb

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