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BCAM Rabbit pAb

211019

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S211019
Product Name:   Anti-BCAM Rabbit Polyclonal Antibody
Synonyms:   AU; LU; CD239; MSK19
UNIPROT ID:   P50895 (Gene Accession - BC050450 )
Background:    This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human BCAM
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 211019(BCAM Antibody) at a dilution of 1/55(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 211019(Anti-BCAM Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 211019(Anti-BCAM Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D122068(Anti-BCAM Antibody) at dilution 1/55.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse kidney tissue, MC3T3-E1 cell lysates;
Primary antibody: 211019(BCAM Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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