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HADHA Rabbit pAb

211322

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S211322
Product Name:   Anti-HADHA Rabbit Polyclonal Antibody
Synonyms:   GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
UNIPROT ID:   P40939 (Gene Accession - BC009235 )
Background:    This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Immunogen:    Fusion protein of human HADHA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 211322(HADHA Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 211322(Anti-HADHA Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: HepG2, Jurkat, 293T, Hela, RAW264.7, K562, NIH/3T3 cell lysates;
Primary antibody: 211322(HADHA Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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