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RASSF7 Rabbit pAb

211478

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S211478
Product Name:   Anti-RASSF7 Rabbit Polyclonal Antibody
Synonyms:   HRC1; HRAS1; C11orf13
UNIPROT ID:   Q02833 (Gene Accession - BC106921 )
Background:    RASSF7 (Ras association domain-containing protein 7), also designated HRAS1-related cluster protein 1, is a 373 amino acid protein that localizes to the centrosome. RASSF7, RASSF8, RASSF9 and RASSF10 are four evolutionary conserved proteins that are collectively known as the N-terminal RASSF family. Expressed in skin, neural tube and eye, RASSF7 is believed to play an essential role in mitosis. Further supporting its mitotic role, knockdown of Xenopus RASSF7 has been shown to influence cell failure to form mitotic spindle and also cause nuclear breakdown and apoptosis.
Immunogen:    Fusion protein of human RASSF7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 211478(RASSF7 Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 211478(Anti-RASSF7 Antibody) at dilution 1/45.

Gel: 8%SDS-PAGE, Lysate: 60 μg;
Lane: Raji cells;
Primary antibody: 211478(RASSF7 Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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