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VPS25 Rabbit pAb

211752

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S211752
Product Name:   Anti-VPS25 Rabbit Polyclonal Antibody
Synonyms:   DERP9; EAP20; FAP20
UNIPROT ID:   Q9BRG1 (Gene Accession - BC006282 )
Background:    This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.
Immunogen:    Fusion protein of human VPS25
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 211752(VPS25 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 211752(Anti-VPS25 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 211752(Anti-VPS25 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D123545(Anti-VPS25 Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse fetal brain tissue, HepG2 cell lysates;
Primary antibody: 211752(VPS25 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
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