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ENO3 Rabbit pAb

212582

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S212582
Product Name:   Anti-ENO3 Rabbit Polyclonal Antibody
Synonyms:   MSE; GSD13
UNIPROT ID:   P13929 (Gene Accession - BC017249 )
Background:    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
Immunogen:    Fusion protein of human ENO3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 212582(ENO3 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 212582(Anti-ENO3 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 212582(Anti-ENO3 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D125431(Anti-ENO3 Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HepG2, NIH/3T3, C2C12, Raji and Jurkat cell lysates;
Primary antibody: 212582(ENO3 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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