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MRPS35 Rabbit pAb

212720

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S212720
Product Name:   Anti-MRPS35 Rabbit Polyclonal Antibody
Synonyms:   MDS023; MRPS28; MRP-S28; HDCMD11P
UNIPROT ID:   P82673 (Gene Accession - BC015862 )
Background:    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.
Immunogen:    Fusion protein of human MRPS35
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 212720(MRPS35 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 212720(Anti-MRPS35 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: LOVO, K562, Jurkat and Hela cell lysates;
Primary antibody: 212720(MRPS35 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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