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XPNPEP3 ? Rabbit pAb

213295

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S213295
Product Name:   Anti-XPNPEP3 Rabbit Polyclonal Antibody
Synonyms:   APP3; NPHPL1
UNIPROT ID:   Q9NQH7 (Gene Accession - NP_071381 )
Background:    The protein encoded by this gene belongs to the family of X-pro-aminopeptidases that utilize a metal cofactor, and remove the N-terminal amino acid from peptides with a proline residue in the penultimate position. This protein has been shown to localize to the mitochondria of renal cells, and have a role in ciliary function. Mutations in this gene are associated with nephronophthisis-like nephropathy-1. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene, however, expression of some of these isoforms in vivo is not known.
Immunogen:    Synthetic peptide of human XPNPEP3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lymphoma tissue using 213295(XPNPEP3 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with the synthetic peptide and then with 213295(Anti-XPNPEP3 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: MCF7 cells, A431 cells;
Primary antibody: 213295(XPNPEP3 Antibody) at dilution 1/100;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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