CREBZF Rabbit pAb

213320

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S213320

Product Name: Anti-CREBZF Rabbit Polyclonal Antibody

Synonyms: ZF; SMILE

UNIPROT ID: Q9NS37 (Gene Accession - NP_001034707 )

Background: Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.

Immunogen: Synthetic peptide of human CREBZF

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Epigenetics and Nuclear Signaling

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213320(CREBZF Antibody) at a dilution of 1/50(Cytoplasm ).	In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213320(Anti-CREBZF Antibody) at dilution 1/50.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-2: Mouse brain tissue, PC3 cells; Primary antibody: 213320(CREBZF Antibody) at dilution 1/500; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 10 seconds

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CREBZF Rabbit pAb

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