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EDA Rabbit pAb

213491

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S213491
Product Name:   Anti-EDA Rabbit Polyclonal Antibody
Synonyms:   ED1, HED, EDA1, EDA2, ODT1, XHED, XLHED, ED1-A1, ED1-A2, STHAGX1
UNIPROT ID:   Q92838 (Gene Accession - NP_001390 )
Background:    The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human EDA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 213491(EDA Antibody) at a dilution of 1/40(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 213491(Anti-EDA Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 213491(Anti-EDA Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D160286(Anti-EDA Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane: Human lung cancer tissue;
Primary antibody: 213491(EDA Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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