IRF1 Rabbit pAb

213613

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S213613

Product Name: Anti-IRF1 Rabbit Polyclonal Antibody

Synonyms: MAR, IRF-1

UNIPROT ID: P10914 (Gene Accession - NP_002189 )

Background: IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Immunogen: Synthetic peptide of human IRF1

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse, Rat

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Epigenetics and Nuclear Signaling, Cardiovascular

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213613(IRF1 Antibody) at a dilution of 1/40(Cytoplasm).	In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213613(Anti-IRF1 Antibody) at dilution 1/40.
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 213613(Anti-IRF1 Antibody) at a dilution of 1/40.	In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D160520(Anti-IRF1 Antibody) at dilution 1/40.
Gel: 12%SDS-PAGE, Lysate: 30 μg; Lane 1-2: NIH/3T3 cells, Jurkat cells; Primary antibody: 213613(IRF1 Antibody) at dilution 1/750; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 1 minute

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