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KLF5 Rabbit pAb

213622

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S213622
Product Name:   Anti-KLF5 Rabbit Polyclonal Antibody
Synonyms:   CKLF; IKLF; BTEB2
UNIPROT ID:   Q13887 (Gene Accession - NP_001721 )
Background:    This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human KLF5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 213622(KLF5 Antibody) at a dilution of 1/120(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 213622(Anti-KLF5 Antibody) at dilution 1/120.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 213622(Anti-KLF5 Antibody) at a dilution of 1/120.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D160534(Anti-KLF5 Antibody) at dilution 1/120.

Gel: 10+12+15%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Jurkat cells, human ovarian cancer tissue;
Primary antibody: 213622(KLF5 Antibody) at dilution 1/1700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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