PRMT5 Rabbit pAb

213719

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S213719

Product Name: Anti-PRMT5 Rabbit Polyclonal Antibody

Synonyms: JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5

UNIPROT ID: O14744 (Gene Accession - NP_001034708 )

Background: Protein arginine methyltransferase 5 has been shown to interact with WD repeat-containing protein 77, CLNS1A, Janus kinase 2, SNRPD3 and SUPT5H.Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation.

Immunogen: Synthetic peptide of human PRMT5

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 15-50;WB: 200-1000;ELISA: 1000-2000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse, Rat

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Epigenetics and Nuclear Signaling

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 213719(PRMT5 Antibody) at a dilution of 1/20(Cytoplasm).	In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 213719(Anti-PRMT5 Antibody) at dilution 1/20.
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 213719(Anti-PRMT5 Antibody) at a dilution of 1/20.	In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D160716(Anti-PRMT5 Antibody) at dilution 1/20.
Gel: 10%SDS-PAGE, Lysate: 40 μg; Lane 1-3: K562 cells, hela cells, 293T cells; Primary antibody: 213719(PRMT5 Antibody) at dilution 1/400; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 2 minutes

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Product Categories

PRMT5 Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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