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NFATC4 Rabbit pAb

213772

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S213772
Product Name:   Anti-NFATC4 Rabbit Polyclonal Antibody
Synonyms:   NFAT3; NF-AT3; NF-ATC4
UNIPROT ID:   Q14934 (Gene Accession - NP_001129494 )
Background:    This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human NFATC4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213772(NFATC4 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213772(Anti-NFATC4 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 213772(NFATC4 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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