SPAG4 Rabbit pAb

213875

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S213875

Product Name: Anti-SPAG4 Rabbit Polyclonal Antibody

Synonyms: SUN4; CT127

UNIPROT ID: Q9NPE6 (Gene Accession - NP_003107 )

Background: The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.

Immunogen: Synthetic peptide of human SPAG4

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 100-300;WB: 200-1000;ELISA: 2000-5000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Signal Transduction

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 213875(SPAG4 Antibody) at a dilution of 1/70(Cytoplasm ).	In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 213875(Anti-SPAG4 Antibody) at dilution 1/70.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 213875(Anti-SPAG4 Antibody) at a dilution of 1/70.	In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D160986(Anti-SPAG4 Antibody) at dilution 1/70.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane: NIH/3T3 cell lysate; Primary antibody: 213875(SPAG4 Antibody) at dilution 1/400; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 5 minutes

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SPAG4 Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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