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ERP29 Rabbit pAb

214316

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S214316
Product Name:   Anti-ERP29 Rabbit Polyclonal Antibody
Synonyms:   ERp28; ERp31; PDIA9; PDI-DB; C12orf8
UNIPROT ID:   P30040 (Gene Accession - NP_006808 )
Background:    This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms
Immunogen:    Synthetic peptide of human ERP29
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 214316(ERP29 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 214316(Anti-ERP29 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 214316(Anti-ERP29 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D161659(Anti-ERP29 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Hela cells, human fetal liver tissue, A549 cells, 293T cells;
Primary antibody: 214316(ERP29 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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