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CENPV Rabbit pAb

214600

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S214600
Product Name:   Anti-CENPV Rabbit Polyclonal Antibody
Synonyms:   p30; PRR6; CENP-V; 3110013H01Rik
UNIPROT ID:   Q7Z7K6 (Gene Accession - NP_859067 )
Background:    Predicted to enable carbon-sulfur lyase activity and metal ion binding activity. Involved in pericentric heterochromatin assembly; positive regulation of cytokinesis; and regulation of chromosome organization. Acts upstream of or within ameboidal-type cell migration. Located in several cellular components, including midbody; nucleus; and spindle midzone.
Immunogen:    Synthetic peptide of human CENPV
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 214600(CENPV Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 214600(Anti-CENPV Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 214600(Anti-CENPV Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D162083(Anti-CENPV Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Raji cell, Hela cell, Jurkat cell, 293T cell, Mouse brain tissue lysate;
Primary antibody: 214600(CENPV Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
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