HIF1AN Rabbit pAb

214616

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S214616

Product Name: Anti-HIF1AN Rabbit Polyclonal Antibody

Synonyms: FIH1

UNIPROT ID: Q9NWT6 (Gene Accession - NP_060372 )

Background: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Immunogen: Synthetic peptide of human HIF1AN

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 50-300;WB: 500-2000;ELISA: 2000-10000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Cardiovascular, Epigenetics and Nuclear Signaling

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 214616(HIF1AN Antibody) at a dilution of 1/40(Cytoplasm and Nucleus).	In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 214616(Anti-HIF1AN Antibody) at dilution 1/40.
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 214616(Anti-HIF1AN Antibody) at a dilution of 1/40.	In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D162109(Anti-HIF1AN Antibody) at dilution 1/40.
Gel: 8%SDS-PAGE, Lysate: 60 μg; Lane 1-2: Mouse brain tissue, Mouse liver tissue; Primary antibody: 214616(HIF1AN Antibody) at dilution 1/200; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 20 seconds

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Product Categories

HIF1AN Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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