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RCC1 Rabbit pAb

214621

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S214621
Product Name:   Anti-RCC1 Rabbit Polyclonal Antibody
Synonyms:   CHC1; RCC1-I
UNIPROT ID:   P18754 (Gene Accession - NP_001041659 )
Background:    Enables several functions, including guanyl-nucleotide exchange factor activity; nucleosomal DNA binding activity; and protein heterodimerization activity. Involved in several processes, including G1/S transition of mitotic cell cycle; regulation of mitotic nuclear division; and spindle organization. Located in chromatin; cytoplasm; and nucleus. Part of protein-containing complex.
Immunogen:    Synthetic peptide of human RCC1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 214621(RCC1 Antibody) at a dilution of 1/50(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 214621(Anti-RCC1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 214621(RCC1 Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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