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SPATA18 Rabbit pAb

214740

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S214740
Product Name:   Anti-SPATA18 Rabbit Polyclonal Antibody
Synonyms:   Mieap; SPETEX1
UNIPROT ID:   Q8TC71 (Gene Accession - NP_660306 )
Background:    SPATA18 (spermatogenesis associated 18), also known as Mieap or SPETEX1, is a 538 amino acid protein that is thought to play a role in cell differentiation during spermatogenesis, particularly during development from late elongate spematids to mature spermatozoa. Localizing to cytoplasm, SPATA18 is encoded by a gene that maps to human chromosome 4q12.
Immunogen:    Synthetic peptide of human SPATA18
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 214740(SPATA18 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 214740(Anti-SPATA18 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human normal stomach tissue;
Primary antibody: 214740(SPATA18 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
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