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SERPINH1 Rabbit pAb

215139

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S215139
Product Name:   Anti-SERPINH1 Rabbit Polyclonal Antibody
Synonyms:   CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
UNIPROT ID:   P50454 (Gene Accession - NP_001226 )
Background:    This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Immunogen:    Synthetic peptide of human SERPINH1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 215139(SERPINH1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 215139(Anti-SERPINH1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 215139(Anti-SERPINH1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D162935(Anti-SERPINH1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A375 and NIH/3T3 cell lysates;
Primary antibody: 215139(SERPINH1 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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