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STK11 Rabbit pAb

215222

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   S215222
Product Name:   Anti-STK11 Rabbit Polyclonal Antibody
Synonyms:   PJS; LKB1; hLKB1
UNIPROT ID:   Q15831 (Gene Accession - NP_000446 )
Background:    This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Immunogen:    Synthetic peptide of human STK11
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 215222(STK11 Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 215222(Anti-STK11 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T, MCF7 cell lysates;
Primary antibody: 215222(STK11 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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