ZNF76 Rabbit pAb

215278

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S215278

Product Name: Anti-ZNF76 Rabbit Polyclonal Antibody

Synonyms: ZNF523; Zfp523; D6S229E

UNIPROT ID: P36508 (Gene Accession - NP_003418 )

Background: ZNF76, also known as ZNF523 or Zfp523, is a transcriptional repressor expressed in the testis. It is the human homolog of the Xenopus Staf protein (selenocysteine tRNA gene transcription-activating factor) known to regulate the genes encoding small nuclear RNA and selenocysteine tRNA. ZNF76 localizes to the nucleus and exerts an inhibitory function on p53-mediated transactivation. ZNF76 specifically targets TFIID (TATA-binding protein). The interaction with TFIID occurs through both its N and C termini. The transcriptional repression activity of ZNF76 is predominantly regulated by lysine modifications, acetylation and sumoylation. ZNF76 is sumoylated by PIAS 1 and is acetylated by p300. Acetylation leads to the loss of sumoylation and a weakened TFIID interaction. ZNF76 can be deacetylated by HDAC1. In addition to lysine modifications, ZNF76 activity is also controlled by splice variants. Two isoforms exist due to alternative splicing. These isoforms vary in their ability to interact with TFIID.

Immunogen: Synthetic peptide of human ZNF76

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse, Rat

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Epigenetics and Nuclear Signaling

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 215278(ZNF76 Antibody) at a dilution of 1/20(Nucleus).	In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 215278(Anti-ZNF76 Antibody) at dilution 1/20.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane: K562 cell lysate; Primary antibody: 215278(ZNF76 Antibody) at dilution 1/200; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 1 minute

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ZNF76 Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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