H3C1 Rabbit pAb

215284

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S215284

Product Name: Anti-H3C1 Rabbit Polyclonal Antibody

Synonyms: H3/A; H3FA; HIST1H3A

UNIPROT ID: P68431 (Gene Accession - NP_003520 )

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Immunogen: Synthetic peptide of human H3C1

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Epigenetics and Nuclear Signaling

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 215284(H3C1 Antibody) at a dilution of 1/30(Nucleus).	In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 215284(Anti-H3C1 Antibody) at dilution 1/30.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 215284(Anti-H3C1 Antibody) at a dilution of 1/30.	In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D163159(Anti-H3C1 Antibody) at dilution 1/30.
Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane 1-7: Hela,231,A549,293T and NIH/3T3 cell,Mouse liver tissue,HUVEC cell lysates; Primary antibody: 215284(H3C1 Antibody) at dilution 1/250; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 70 seconds

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H3C1 Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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