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VRK2 Rabbit pAb

215327

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Product Description Reference citations(0) Related Products
Cat.#:   S215327
Product Name:   Anti-VRK2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q86Y07 (Gene Accession - NP_006287 )
Background:    This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity.
Immunogen:    Synthetic peptide of human VRK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 35-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 215327(VRK2 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 215327(Anti-VRK2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 215327(Anti-VRK2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D163214(Anti-VRK2 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal liver tissue lysate;
Primary antibody: 215327(VRK2 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 seconds
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