LDHB Rabbit pAb

222426

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S222426

Product Name: Anti-LDHB Rabbit Polyclonal Antibody

Synonyms: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281

UNIPROT ID: P07195 (Gene Accession - NP_002291 )

Background: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes ,x 5 and 13.

Immunogen: Synthetic peptide of human LDHB

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse, Rat

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Metabolism, Cancer

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222426(LDHB Antibody) at a dilution of 1/50(Cytoplasm).	In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222426(Anti-LDHB Antibody) at dilution 1/50.
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 222426(Anti-LDHB Antibody) at a dilution of 1/50.	In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D264590(Anti-LDHB Antibody) at dilution 1/50.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-7: 293T, Jurkat, A549, Hela, Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue lysates; Primary antibody: 222426(LDHB Antibody) at dilution 1/800; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 5 seconds

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Product Categories

LDHB Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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