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ATP Citrate Lyase (3D9) Mouse mAb

261031

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261031
Product Name:   Anti-ATP Citrate Lyase (3D9) Mouse Monoclonal Antibody
Synonyms:   ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme
UNIPROT ID:   P53396
Background:    ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.
Immunogen:    Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,FC
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 FC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    3D9-E9-H8
MW:    Calculated MW: 121 kDa; Observed MW: 121 kDa
Isotype:    IgG2a
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Monkey
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of ATP Citrate Lyase in HeLa cells using ATPCitrate Lyase (Cterminus) antibody.

Western blot analysis of ATPCitrate Lyase in 3T3, K562, COS7 and Hela lysates using ATPCitrate Lyase antibody.

Flow Cytometry analysis of HeLa cells stained with ATPCitrate Lyase (red). Black line histogram represents the isotype control, normal mouse IgG
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