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Aurora A (2E3) Mouse mAb

261042

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261042
Product Name:   Anti-Aurora A (2E3) Mouse Monoclonal Antibody
Synonyms:   AIK; ARK1; AYK1; Aurora-A; Aurora-related kinase 1; BTAK; IAK1; Ipl1- and aurora-related kinase 1; STK15; STK6; Serine/threonine kinase 15
UNIPROT ID:   O14965
Background:    Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis.
Immunogen:    Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli.
Applications:    WB,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    2E3-C10-C11-G6
MW:    Calculated MW: 46 kDa; Observed MW: 46 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Monkey
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone phosphorylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Aurora A (2E3) in HeLa using Aurora Kinase A antibody.

Western blot analysis of Aurora Kinase A in SW480 and COS7 lysates using Aurora Kinase A antibody.

Immunofluorescence analysis of Aurora A (2E3) in Hela cells using Aurora Kinase A antibody(green) and Histone H3.1 (Phospho-Ser1) antibody (red),and DAPI (blue)
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