ZAP70 (4B2) Mouse mAb

261113

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N261113

Product Name: Anti-ZAP70 (4B2) Mouse Monoclonal Antibody

Synonyms: ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

UNIPROT ID: P43403

Background: The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.

Immunogen: Purified recombinant human ZAP-70 protein fragments expressed in E.coli.

Applications: WB,IP

Recommended Dilutions: WB: 1/500-1/1000 IP: 1/20

Host Species: Mouse

Clonality: Mouse Monoclonal

Clone ID: 4B2-C1-H3

MW: Calculated MW: 70 kDa; Observed MW: 70 kDa

Isotype: IgG2b

Purification: Affinity Purified

Species Reactivity: Human

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Immunology

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of ZAP70 (4B2) in Jurkat lysates (ZAP70 positive expression cell line)and Ramos lysates(ZAP70 negative expression cell line) using ZAP70 antibody.

Western blot analysis of ZAP70 in CEM and Jurkat lysates, negative in the Ramos lysates using ZAP70 antibody.

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ZAP70 (4B2) Mouse mAb

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