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Rabbit IgG (5B2) Mouse mAb

261136

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261136
Product Name:   Anti-Rabbit IgG (5B2) Mouse Monoclonal Antibody
Synonyms:   Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS.
UNIPROT ID:   -
Background:    Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes.
Immunogen:    Rabbit IgG
Applications:    WB,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 ELISA: 1/10000
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    5B2-F9-D6
MW:    Calculated MW: 55,25 kDa; Observed MW: 55,25 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Rabbit
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of Rabbit IgG (5B2) in CHO-K1 lysates transfected with HAtagged NGAL using HATag antibody (lane 2). Lane 1 contains rabbit IgG IP control lysates. All lanes are probed with HATag antibody as the primary antibody. Panel A incubated with secondary antibodies anti-rabbit whole IgG, HRPlinked antibody for detecting.Panel B incubated with anti-rabbit IgG (HeavyChain Specific) mAb and the Anti-mouse whole IgG, HRPlinked antibody was used for detecting.

Western blot analysis of MEK2 in THP-1, Jurkat, Raji, 3T3 and Hela lysates using MEK2 antibody, then incubated with N261136(Rabbit IgG mAb).Anti-mouse IgG, HRPlinked antibody was used for detecting.

Western blot analysis of Rabbit IgG (5B2) in decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using IgG (HeavyChain Specific)antibody.
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