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E2F1 (4G8) Mouse mAb

261140

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261140
Product Name:   Anti-E2F1 (4G8) Mouse Monoclonal Antibody
Synonyms:   E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
UNIPROT ID:   Q01094
Background:    The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
Immunogen:    Purified recombinant human E2F-1 protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4G8-2C2-C12
MW:    Calculated MW: 47 kDa; Observed MW: 70 kDa
Isotype:    IgG2b
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of E2F1 (4G8) in Hela using E2F1 antibody,and DAPI (blue).

Western blot analysis of E2F1 in C6, Raw264.7 and Hela lysates using E2F1 antibody.

Immunoprecipitation analysis of E2F1 (4G8) in Hela lysates using E2F1 antibody.
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