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eIF2A (2E4F5) Mouse mAb

261144

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261144
Product Name:   Anti-eIF2A (2E4F5) Mouse Monoclonal Antibody
Synonyms:   EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
UNIPROT ID:   P05198
Background:    eIF2A a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.
Immunogen:    Purified recombinant human eIF2α protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    2E4-F5-E7
MW:    Calculated MW: 36 kDa; Observed MW: 36 kDa
Isotype:    IgG2b
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of eIF2A (2E4F5) in Hela using eIF2???¤ antibody.

Western blot analysis of eIF2???¤ in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela lysates using eIF2???¤ antibody.

Immunoprecipitation analysis of eIF2A (2E4F5) in Hela lysates using eIF2???¤ antibody.
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