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Vimentin (4F8) Mouse mAb

261147

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261147
Product Name:   Anti-Vimentin (4F8) Mouse Monoclonal Antibody
Synonyms:   VIM; Vimentin
UNIPROT ID:   P08670
Background:    Vimentin an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Immunogen:    Purified recombinant human Vimentin protein fragments expressed in E.coli.
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4F8-A5-H5
MW:    Calculated MW: 54 kDa; Observed MW: 57 kDa
Isotype:    IgG2a
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Vimentin (4F8) in Hela using Vimentin antibody.

Western blot analysis of Vimentin in Molt4, K562, COS7, Jurkat, Hela and Vimentin negative (Daudi, Ramos, Raji) lysates using Vimentin antibody.

Immunoprecipitation analysis of Vimentin (4F8) in Hela lysates using Vimentin antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsils using Vimentin (4F8) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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