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E Cadherin (6B10) Mouse mAb

261162

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261162
Product Name:   Anti-E Cadherin (6B10) Mouse Monoclonal Antibody
Synonyms:   CDH1; CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD antigen CD324
UNIPROT ID:   P12830
Background:    CDH1 a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells.
Immunogen:    A synthesized peptide derived from human E Cadherin
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    6B10-F4-G10
MW:    Calculated MW: 97 kDa; Observed MW: 135 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsils using ECadherin (6B1) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of ECadherin in T47D, HCT116, MDA-MB-468 and Caco2 lysates using ECadherin antibody.

Western blot analysis of E Cadherin (6B10) in mouse embryos and HCT116 lysates using ECadherin antibody.
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