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NCK1 (7H2) Mouse mAb

261201

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N261201
Product Name:   Anti-NCK1 (7H2) Mouse Monoclonal Antibody
Synonyms:   NCK; nck-1; NCKalpha
UNIPROT ID:   P16333
Background:    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Immunogen:    Purified recombinant fragment of human NCK1 expressed in E. Coli.
Applications:    IHC-P,FC
Recommended Dilutions:    IHC: 1/50-1/100 FC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    7H2-4C2-1A2
MW:    -
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Monkey
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded rectum cancer tissues using NCK1 antibody with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of NCK1 (7H2) in Human NCK1 (AA: 20337recombinant protein. ) using NCK1 (7H2) antibody.

Western blot analysis of NCK1 (7H2) in Jurkat , HeLa , HEK293 , A431 , K562 ,and COS7 lysates using NCK1 (7H2) antibody

Flow cytometry analysis of Jurkat stained with NCK1 antibody (green) and negative control (red).
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